• Catalog Number:

  E1A8133A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Stathmin around the phosphorylation site of serine 439 (R-R-SP-I-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O43318

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  Mitogen-activated protein kinase kinase kinase 7; EC=2.7.11.25; MAP3K7; TAK1

• Specificity Sensitivity:

  MAP3K7 (Phospho-Ser439) antibody detects endogenous levels of MAP3K7 only when phosphorylated at serine 439.

• References:

  Sakurai H., Biochem. Biophys. Res. Commun. 243:545-549(1998). Mungall A.J., Nature 425:805-811(2003). Shibuya H., Science 272:1179-1182(1996).

• All Sites:

  Human: Ser439; Mouse: Ser412; Rat: Ser439

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K7 (Phospho-Ser439) antibody. Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using MAP3K7 (Phospho-Ser439) antibody.