• Catalog Number:

  E1A8129A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MAP3K1 around the phosphorylation site of threonine 1402 (K-G-TP-G-A).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q13233

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  EC 2.7.11.25; kinase MEKK1; M3K1; MAP3K1; MAPK/ERK kinase kinase 1; MAPKKK1; MEK kinase 1; MEKK; MEKK 1; MEKK1; mitogen-activated protein kinase kinase kinase 1

• Specificity Sensitivity:

  MAP3K1 (Phospho-Thr1402) antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.

• References:

  Schmutz J., Nature 431:268-274(2004). Xia Y., Genes Dev. 12:3369-3381(1998). Vinik B.S., Mamm. Genome 6:782-783(1995). - ---------------------------------------------------------------------------------------------------------------------------------------------------------------

• All Sites:

  Human: Thr1402; Mouse: Thr1381; Rat: Thr1381

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) antibody. Western blot analysis of extracts from Jurkat cells, using MAP3K1 (Phospho-Thr1402) antibody.