• Catalog Number:

  E1A8118A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 931 (M-E-YP-L-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  O60674

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  EC 2.7.10.2; JAK-2; JAK2; Janus kinase 2; kinase Jak2

• Specificity Sensitivity:

  JAK2 (Phospho-Tyr931) antibody detects endogenous levels of JAK2 only when phosphorylated at tyrosine 931.

• References:

  Saltzman A., Biochem. Biophys. Res. Commun. 246:627-633(1998). Dalal I., Blood 91:844-851(1998). Peeters P., Blood 90:2535-2540(1997).

• All Sites:

  Human: Tyr931; Mouse: Tyr931; Rat: Tyr931

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using JAK2 (Phospho-Tyr931) antibody. Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3mM, 40mins), using JAK2 (Phospho-Tyr931) antibody.