• Catalog Number:

  E1A8112A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ICK around the phosphorylation site of tyrosine 159(T-D-YP-V-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  Q9UPZ9

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  EC 2.7.11.22; hICK; ICK; Intestinal cell kinase; KIAA0936; kinase ICK; Laryngeal cancer kinase 2; LCK2; MAK-related kinase; MRK; Serine/threonine kinase ICK

• Specificity Sensitivity:

  ICK (Phospho-Tyr159) antibody detects endogenous levels of ICK only when phosphorylated at tyrosine 159.

• References:

  Togawa K., J. Cell. Physiol. 183:129-139(2000). Yang T., Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases. Nagase T., DNA Res. 6:63-70(1999).

• All Sites:

  Human: Tyr159; Mouse: Tyr159; Rat: Tyr159

Western blot analysis of extracts from 3T3 cells, treated with starved (24hours), using ICK (Phospho-Tyr159).