• Catalog Number:

  E1A8107A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human FLT3 around the phosphorylation site of tyrosine 842 (S-N-YP-V-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 84%, Positives = 100%)

• SwissProtNo:

  P36888

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  CD135; EC 2.7.10.1; fetal liver kinase 2; FL cytokine receptor; FLK-2; FLT-3; FLT3; fms-related tyrosine kinase 3; kinase Flt3; stem cell tyrosine kinase 1; STK-1; STK1

• Specificity Sensitivity:

  FLT3 (Phospho-Tyr842) antibody detects endogenous levels of FLT3 only when phosphorylated at tyrosine 842.

• References:

  Small D., Proc. Natl. Acad. Sci. U.S.A. 91:459-463(1994). Rosnet O., Blood 82:1110-1119(1993). Rosnet O., Genomics 9:380-385(1991).

• All Sites:

  Human: Tyr842; Mouse: Tyr845

Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using FLT3 (Phospho-Tyr842)) antibody.