• Catalog Number:

  E1A8095A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human EPHA3/4/5 around the phosphorylation site of tyrosine 779 (A-A-YP-T-T).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P29320/54764/54756

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  EC 2.7.10.1; EPA3; ephrin type-A receptor 3; ETK; ETK1; HEK; HEK4; kinase EphA3; MEK4; REK4; TYRO-4; TYRO4; tyrosine-protein kinase receptor ETK1; tyrosine-protein kinase receptor REK4

• Specificity Sensitivity:

  EPHA3/4/5 (Phospho-Tyr779/833) antibody detects endogenous levels of EPHA3/4/5 only when phosphorylated at tyrosine 779.

• References:

  Wicks I.P., Proc. Natl. Acad. Sci. U.S.A. 89:1611-1615(1992). Chiari R., Cancer Res. 60:4855-4863(2000). Boyd A.W., J. Biol. Chem. 267:3262-3267(1992).

• All Sites:

  Human: Tyr779/779/833; Mouse: Tyr779/779/672; Rat: Tyr780/-/835

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using EPHA3/4/5 (Phospho-Tyr779/833) antibody. EPHA3/4/5 (Phospho-Tyr779/833) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.