• Catalog Number:

  E1A8088A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CK-1α around the phosphorylation site of tyrosine 294 (Y-D-YP-T-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P48729/Q8N752

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Casein kinase I, alpha isoform; CK1-alpha; CKI-alpha; CSNK1A1; EC 2.7.11.1; KC1A; kinase CK1-alpha

• Specificity Sensitivity:

  CK-1α (Phospho-Tyr294) antibody detects endogenous levels of CK-1α only when phosphorylated at tyrosine 294.

• References:

  Tapia C., FEBS Lett. 349:307-312(1994). Fish K.J., J. Biol. Chem. 270:14875-14883(1995). Halleck A., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

• All Sites:

  Human: Tyr294; Mouse: Tyr294; Rat: Tyr294

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using CK-1α (Phospho-Tyr294) antibody. Western blot analysis of extracts from HT-29 cells, treated with heat shock, using CK-1α (Phospho-Tyr294) antibody.