• Catalog Number:

  E1A8074A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human ABL1 around the phosphorylation site of threonine 735 (S-V-TP-L-P).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P00519

• Applications:

  WB: 1:500~1:1000 ELISA: 1:1000

• Other Names:

  Abelson murine leukemia viral oncogene 1; ABL1; c-ABL; EC 2.7.10.2; JTK7; p150; proto-oncogene tyrosine-protein kinase ABL1; v-abl Abelson murine leukemia viral oncogene 1

• Specificity Sensitivity:

  ABL1 (Phospho-Thr735) antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735.

• References:

  Fainstein E., Oncogene 4:1477-1481(1989). Shtivelman E., Cell 47:277-284(1986). Chissoe S.L., Genomics 27:67-82(1995).

• All Sites:

  Human: Thr735; Mouse: Thr734; Rat: Thr753

Western blot analysis of extracts from COS cells, treated with EGF (200ng/ml, 30mins), using ABL1 (Phospho-Thr735) antibody.