Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Retinoblastoma around the phosphorylation site of serine 788 (P-R-SP-P-Y).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06400
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Other Names:
P105-RB; PP105; PP110; RB-1; RB1; retinoblastoma gene; Retinoblastoma-associated protein
Specificity Sensitivity:
Retinoblastoma (Phospho-Ser788) antibody detects endogenous levels of Retinoblastoma only when phosphorylated at serine 788.
References:
Lee W.-H., Nature 329:642-645(1987).
Friend S.H., Proc. Natl. Acad. Sci. U.S.A. 84:9059-9063(1987).
McGee T.L., Gene 80:119-128(1989).
All Sites:
Human: Ser788; Mouse: Ser781; Rat: Ser759
P-peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Retinoblastoma (Phospho-Ser788) antibody.