• Catalog Number:

  E1A8045A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of serine 283 (R-R-SP-L-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P53671

• Applications:

  IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  EC 2.7.11.1; kinase LIMK2; LIM domain kinase 2; LIMK-2

• Specificity Sensitivity:

  LIMK2 (Phospho-Ser283) antibody detects endogenous levels of LIMK2 only when phosphorylated at serine 283.

• References:

  Okano I., J. Biol. Chem. 270:31321-31330(1995). Osada H., Biochem. Biophys. Res. Commun. 229:582-589(1996). Dunham I., Nature 402:489-495(1999).

• All Sites:

  Human: Ser283; Mouse: Ser283; Rat: Ser283

P-peptide - + Immunohistochemistry analysis of paraffin-embedded human brain tissue using LIMK2 (Phospho-Ser283) antibody.