• Catalog Number:

  E1A8040A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of threonine 142 (L-L-TP-D-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%)

• SwissProtNo:

  Q00613

• Applications:

  IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1; HSTF1

• Specificity Sensitivity:

  HSF1 (Phospho-Thr142) antibody detects endogenous levels of HSF1 only when phosphorylated at threonine 142.

• References:

  Rabindran S.K., Proc. Natl. Acad. Sci. U.S.A. 88:6906-6910(1991). The MGC Project Team; Genome Res. 14:2121-2127(2004). Schuetz T.J.,Proc. Natl. Acad. Sci. U.S.A. 88:6911-6915(1991).

• All Sites:

  Human: Thr142; Mouse: Thr142

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using HSF1 (Phospho-Thr142) antibody. HSF1 (Phospho-Thr142) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.