• Catalog Number:

  E1A8039A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 1112(Q-R-YP-S-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  P04626

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  C-erbB-2; EC 2.7.10.1; Epidermal growth factor receptor-related protein; ErbB2; HER2; kinase ErbB2; MLN 19; NEU; NEU proto-oncogene; NGL; p185erbB2; Receptor protein-tyrosine kinase erbB-2; Receptor protein-tyrosine kinase erbB-2 precursor; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)

• Specificity Sensitivity:

  HER2 (Phospho-Tyr1112) antibody detects endogenous levels of HER2 only when phosphorylated at serine 385.

• References:

  Yamamoto T., Nature 319:230-234(1986). Coussens L., Science 230:1132-1139(1985). Tal M., Mol. Cell. Biol. 7:2597-2601(1987).

• All Sites:

  Human: Tyr1112; Mouse: Tyr1113; Rat: Tyr1114

Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 20mins), using HER2 (Phospho-Tyr1112) antibody.