• Catalog Number:

  E1A8038A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human GR around the phosphorylation site of serine 203 (S-G-SP-P-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 93%, Positives = 100%)

• SwissProtNo:

  P04150

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  GCR; glucocorticoid receptor; GRL; GRL1; NR3C1

• Specificity Sensitivity:

  GR (Phospho-Ser203) antibody detects endogenous levels of GR only when phosphorylated at serine 203.

• References:

  Hollenberg S.M., Nature 318:635-641(1985). Encio I.J., J. Biol. Chem. 266:7182-7188(1991). Munroe D.G., Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases. ----------------------------------------------------------------------------

• All Sites:

  Human: Ser203; Mouse: Ser202

Western blot analysis of extracts from A549 cells, treated with dexamethason (10nM, 1hour), using GR (Phospho-Ser203) antibody.