• Catalog Number:

  E1A8030A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 146 (S-G-YP-L-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 100%);Rat (Identities = 92%, Positives = 100%)

• SwissProtNo:

  P15311

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Cytovillin; EZRI; p81; VIL2; Villin 2

• Specificity Sensitivity:

  Ezrin (Phospho-Tyr146) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 146.

• References:

  Gould K.L., EMBO J. 8:4133-4142(1989). Bechtel S., BMC Genomics 8:399-399(2007). Mungall A.J., Nature 425:805-811(2003).

• All Sites:

  Human: Tyr146; Mouse: Tyr146; Rat: Tyr146

Western blot analysis of extracts from 293 cells, treated with UV (15mins), using Ezrin (Phospho-Tyr146) antibody.