• Catalog Number:

  E1A8017A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25A around the phosphorylation site of serine 178 (Q-N-SP-A-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 92%, Positives = 92%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  P30304

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  CDC25M3; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1

• Specificity Sensitivity:

  CDC25A (Phospho-Ser178) antibody detects endogenous levels of CDC25A only when phosphorylated at serine 178.

• References:

  Galaktionov K.I., Cell 67:1181-1194(1991). Wegener S., Eur. J. Cell Biol. 79:810-815(2000). Zhao H., Proc. Natl. Acad. Sci. U.S.A. 99-14795-14800(2002).

• All Sites:

  Human: Ser178; Mouse: Ser171; Rat: Ser179

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using CDC25A (Phospho-Ser178) antibody. Western blot analysis of extracts from A549 cells, treated with UV (15mins), using CDC25A (Phospho-Ser178) antibody.