• Catalog Number:

  E1A8016A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human CDC25A around the phosphorylation site of threonine 507.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human; Mouse; Rat

• SwissProtNo:

  P30304

• Applications:

  WB: 1:500~1:3000 ELISA: 1:10000

• Other Names:

  CDC25A; CDC25A2; cell division cycle 25 homolog A (S. pombe); cell division cycle 25A; Dual specificity phosphatase Cdc25A; M-phase inducer phosphatase 1; MPIP1

• Specificity Sensitivity:

  CDC25A (Phospho-Thr507) antibody detects endogenous levels of CDC25A only when phosphorylated at threonine 507.

• References:

  Galaktionov K.I., Cell 67:1181-1194(1991). Varmeh-Ziaie S., Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases. Wegener S., Eur. J. Cell Biol. 79:810-815(2000).

• All Sites:

  Human: Thr507; Mouse: Thr497; Rat: Thr508

Western blot analysis of extracts from HuvEc cells, using CDC25A (Phospho-Thr507) antibody.