• Catalog Number:

  E1A8011A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Caspase 8 around the phosphorylation site of tyrosine380 (Q-P-YP-L-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%)

• SwissProtNo:

  Q14790

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Other Names:

  Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; Caspase-8 precursor; EC 3.4.22.-; EC 3.4.22.61; FADD-homologous ICE/CED-3- like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; ICE8; MACH; MCH5; MORT1-associated CED-3 homolog

• Specificity Sensitivity:

  Caspase 8 (Phospho-Tyr380) antibody detects endogenous levels of Caspase 8 only when phosphorylated at tyrosine 380.

• References:

  Boldin M.P.,Cell 85:803-815(1996). Muzio M., Cell 85:817-827(1996). Fernandes-Alnemri T.,Proc. Natl. Acad. Sci. U.S.A. 93:7464-7469(1996).

• All Sites:

  Human: Tyr380

Western blot analysis of extracts from LOVO cells, treated with UV (15mins), using Caspase 8 (Phospho-Tyr380) antibody. Caspase 8 (Phospho-Tyr380) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.