• Catalog Number:

  E1A8009A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Catenin-β around the phosphorylation site of tyrosine 489 (L-H-YP-G-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P35222

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  catenin (cadherin-associated protein), beta 1; catenin, beta; CATNB; CTNB1; CTNNB

• Specificity Sensitivity:

  Catenin-β (Phospho-Tyr489) antibody detects endogenous levels of Catenin-β only when phosphorylated at tyrosine 489.

• References:

  Huelsken J., J. Cell Biol. 127:2061-2069(1994). Zhang C., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases. Kim J.-S., Cancer Res. 62:2744-2748(2002).

• All Sites:

  Human: Tyr489; Mouse: Tyr489; Rat: Tyr489

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Catenin-β (Phospho-Tyr489) antibody. Western blot analysis of extracts from COS cells, treated with UV (15mins), using Catenin-β (Phospho-Tyr489) antibody.