• Catalog Number:

  E1A7254A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 951 (K-D-YP-V-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35968

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  VEGFR2 (phospho-Tyr951) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 951.

• References:

  Zeng H, et al. (2001) J Biol Chem. 276(35): 32714-32719. Dougher M, et al. (1999) Oncogene. 18(8): 1619-1627.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using VEGFR2 (phospho-Tyr951) antibody.

Western blot analysis of extracts from SK-OV3 cells using VEGFR2 (Ab-951) antibody (Line 1 and 2) and VEGFR2 (phospho-Tyr951) antibody (Line 3 and 4).