• Catalog Number:

  E1A7252A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human VEGFR2 around the phosphorylation site of tyrosine 1175 (K-D-YP-I-V).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P35968

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  EC 2.7.10.1; FLK1; KDR; kinase insert domain receptor; protein-tyrosine kinase receptor Flk-1; vascular endothelial growth factor receptor 2; VGFR2; VGR2

• Specificity Sensitivity:

  VEGFR2 (Phospho-Tyr1175) antibody detects endogenous levels of VEGFR2 only when phosphorylated at tyrosine 1175.

• References:

  Yin L.Y., Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases. Yu Y., Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases. Terman B.I., Oncogene 6:1677-1683(1991).

• All Sites:

  Human: Tyr1175; Mouse: Tyr1173; Rat: Tyr1171

Western blot analysis of extracts from NIH-3T3 cells and HUVEC cells treated with EGF (200ng/ml, 30mins), using VEGFR2 (Phospho-Tyr1175) antibody.