• Catalog Number:

  E1A7251A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P15498

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  Vav (phospho-Tyr174) antibody detects endogenous levels of Vav only when phosphorylated at tyrosine 174.

• References:

  Amarasinghe GK, et al. (2005) Biochemistry; 44(46): 15257-68. Charvet C, et al. (2005) J Biol Chem; 280(15): 15289-99. Miura-Shimura Y, et al. (2003) J Biol Chem; 278(40): 38495-504. Kuhne MR, et al. (2000) J Biol Chem; 275(3): 2185-90.

Western blot analysis of the extracts from Jurkat cells using Vav (phospho-Tyr174) antibody.