Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Tau around the phosphorylation site of Serine 404(D-T-SP-P-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
Tau (Phospho-Ser404) Antibody detects endogenous levels of Tau only when phosphorylated at Serine404.
References:
Li G, Yin H, et.al .(2004) J Biol Chem ; 279(16): 15938-45.
Noble W, et al. (2003) Neuron ; 38(4): 555-65.
Giasson BI, et al.(2002).. Biochemistry; 41(51): 15376-87.
Lee G., et.al. (1989). Neuron 2:1615-1624.
Andreadis A.,et.al. (1992) Biochemistry 31:10626-10633.
Western blot analysis of extracts from mouse brain tissue using Tau (Ab-404) Antibody and Tau (Phospho-Ser404) Antibody.