Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Synuclein-α around the phosphorylation site of tyrosine 136 (Q-D-YP-E-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P37840
Applications:
WB: 1:500~1:1000 ELISA: 1:5000
Other Names:
alpha-synuclein; NACP; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; SYN; synuclein, alpha; SYUA
Specificity Sensitivity:
Synuclein-α (Phospho-Tyr136) antibody detects endogenous levels of Synuclein-α only when phosphorylated at tyrosine 136.
References:
Ueda K., Proc. Natl. Acad. Sci. U.S.A. 90:11282-11286(1993).
Campion D., Genomics 26:254-257(1995).
Ueda K., Biochem. Biophys. Res. Commun. 205:1366-1372(1994).
Western blot analysis of extracts from mouse brain cells, using Synuclein-α (Phospho-Tyr136) antibody.