• Catalog Number:

  E1A7221A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human STAT1 around the phosphorylation site of serine 727 (P-M-SP-P-E).

• Purification:

  The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P42224

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  Signal transducer and activator of transcription 1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

• Specificity Sensitivity:

  STAT1 (Phospho-Ser727) antibody detects endogenous levels of STAT1 only when phosphorylated at serine 727.

• References:

  Schindler C., Proc. Natl. Acad. Sci. U.S.A. 89:7836-7839(1992). Livingston R.J., Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases. Hillier L.W., Nature 434:724-731(2005).

• All Sites:

  Human: Ser727; Mouse: Ser727; Rat: Ser727

P-peptide - +

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using STAT1 (Phospho-Ser727) antibody. Western blot analysis of extracts from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) antibody.