• Catalog Number:

  E1A7216A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human SEK1/MKK4 around the phosphorylation site of threonine261 (A-K-TP-R-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P45985

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Other Names:

  c-Jun N- terminal kinase kinase 1; C-JUN N-terminal kinase kinase 1; Dual specificity mitogen-activated protein kinase kinase 4; EC 2.7.12.2; JNK activating kinase 1; JNK kinase 1; JNKK; JNKK 1; JNKK1; kinase SEK1; MAP kinase kinase 4; MAP2K4; MAPK/ERK kinase 4; MAPKK 4; MKK4; MP2K4; PRKMK4; SAPK/ERK kinase 1; SEK1; SERK1

• Specificity Sensitivity:

  SEK1/MKK4 (Phospho-Thr261) antibody detects endogenous levels of SEK1/MKK4 only when phosphorylated at threonine 261.

• References:

  Lin A., Science 268:286-290(1995). Derijard B., Science 267:682-685(1995). Su G.H., Cancer Res. 58:2339-2342(1998).

• All Sites:

  Human: Thr261; Mouse: Thr259; Rat: Thr259

Western blot analysis of extracts from 293 cells, treated with UV (15mins), using SEK1/MKK4 (Phospho-Thr261) antibody.