• Catalog Number:

  E1A7214A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 235 (R-L-SP-S-L).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P62753

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6, ribosomal protein S6

• Specificity Sensitivity:

  S6 Ribosomal protein (phospho-Ser235) antibody detects endogenous levels of S6 Ribosomal protein only when phosphorylated at serine 235.

• References:

  McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081. Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097. Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222. Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.

• All Sites:

  Human: Ser235; Mouse: Ser235; Rat: Ser235

Western blot analysis of the extracts from 293 cells untreated or treated with serum (10%, 15min), using S6 Ribosomal protein (Ab-235) antibody (Line 1 and 2) and S6 Ribosomal protein (phospho-Ser235) antibody (Line 3 and 4).