Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of Serine 380.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P60484
Applications:
IHC: 1:50~1:100 ELISA: 1:10000
Specificity Sensitivity:
PTEN (Phospho-Ser380) Antibody detects endogenous levels of PTEN only when phosphorylated at Serine 380.
References:
Al-Khouri AM, et al. (2005). J Biol Chem.280(42):35195-35202.
Torres J, et al. (2001). J Biol Chem.276(2): 993-998.
Vazquez F, et al. (2000). Mol Cell Biol.20(14): 5010-5018.
Immunohistochemical analysis of paraffin-embedded breast carcinoma, using PTEN (Phospho-Ser380) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.