• Catalog Number:

  E1A7201A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PTEN around the phosphorylation site of serine 370 (D-V-SP-D-N).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P60484

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  PTEN (phospho-Ser370) antibody detects endogenous levels of PTEN only when phosphorylated at serine 370.

• References:

  Al-Khouri AM, et al. (2005). J Biol Chem.280 (42):35195-35202. Miller SJ, et al. (2002). FEBS Lett. 528(1-3): 145-153. Torres J, et al. (2001). J Biol Chem.276 (2): 993-998. Vazquez F, et al. (2000). Mol Cell Biol.20 (14): 5010-5018.

P-Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PTEN (phospho-Ser370) antibody.

Western blot analysis of extracts from HeLa cells using PTEN (phospho-Ser370) antibody.