Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Progesterone Receptor around the phosphorylation site of Serine 190 (G-L-SP-P-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Reactivity:
Human
SwissProtNo:
P06401
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
Progesterone Receptor (Phospho-Ser190) Antibody detects endogenous levels of Progesterone Receptor only when phosphorylated at Serine 190.
Narayanan R, et al. (2005) Mol Cell Biol; 25(8): 2885-98.
Knotts TA, et al. (2001) J Biol Chem; 276(11): 8475-83.
Clemm DL, et al. (2000) Mol Endocrinol; 14(1): 52-65.