• Catalog Number:

  E1A7196A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human PKCδ around the phosphorylation site of serine 645 (R-L-SP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q05655

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  PRKCD, Protein kinase C delta type

• Specificity Sensitivity:

  PKCδ (phospho-Ser645) antibody detects endogenous levels of PKCδ only when phosphorylated at serine 645.

• References:

  Kei Sakamoto, et,al. (2003) Am J Physiol Endocrinol Metab; 285: E1081-E1088. Ling Zhang, et,al. (2004) J. Biol. Chem; 279: 28315-28319. Kristof Van Kolen et,al. (2006) FEBS J; 273: 1843-1854. Martin Villalba, et,al. (2002) J. Cell Biol; 157: 253.

• All Sites:

  Human: Ser645; Mouse: Ser643; Rat: Ser643

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (phospho-Ser645) antibody.

Western blot analysis of extracts from MCF7 cells, using PKCδ (Ab-645) antibody (Line 1 and 2) and PKCδ (phospho-Ser645) antibody (Line 3 and 4).