• Catalog Number:

  E1A7190A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of threonine 421(P-R-TP-P-V). The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  S6K1; p70(S6K)-ALPHA; p70-ALPHA, p70 S6 KINASE ALPHA, ribosomal protein S6 kinase 70kDa, polypeptide 1

• Specificity Sensitivity:

  p70 S6 Kinase (phospho-Thr421) antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at threonine 421.

• References:

  BA Moser, Mol. Cell. Biol., Sep 1997; 17: 5648 - 5655. Nicholas Lehman, FASEB J, Apr 2007; 21: 1075 - 1087. Bernard Masri, FASEB J, Sep 2004; 10.1096/fj.04-1930fje. Julian Gomez-Cambronero, J. Immunol., Dec 2003; 171: 6846.

• All Sites:

  Human: Thr421; Mouse: Thr444; Rat: Thr421

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (phospho-Thr421) antibody.

Western blot analysis of extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 30mins), using p70 S6 Kinase (phospho-Thr421) antibody (Line 1 and 2).