• Catalog Number:

  E1A7189A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-SP-P-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P23443

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  Ribosomal protein S6 kinase I; S6K; S6K1; 70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase alpha; p70(S6K)-alpha; p70-S6K; P70S6K; p70-alpha

• Specificity Sensitivity:

  p70 S6 Kinase (Phospho-Ser424) antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at serine 424.

• References:

  Philip J. Papst, et al. (1998) J. Biol. Chem; 273: 15077. Satoru Eguchi, et al. (999) J. Biol. Chem 1; 274: 36843. Ana González-García, et al. (2002) J. Biol. Chem; 277: 1500-1508. Ulrike Krause, et al. (2002) Eur. J. Biochem; 269: 3751.

• All Sites:

  Human: Ser424; Mouse: Ser424; Rat: Ser424

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser424) antibody.

Western blot analysis of extracts from 293 cells, untreated or treated with serum(15%), using p70 S6 Kinase (Ab-424) antibody (Line 1 and 2) and p70 S6 Kinase (Phospho-Ser424) antibody (Line 3 and 4).