• Catalog Number:

  E1A7187A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of threonine18 (Q-E-TP-F-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  p53 (phospho-Thr18) antibody detects endogenous levels of p53 only when phosphorylated at threonine 18.

• References:

  Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Erker L, et al. (2005) Hum Mol Genet. 14(12): 1699-1708. Jones RG, et al. (2005) Mol Cell.18 (3): 283-293. Zhang X, et al. (2005) J Biol Chem. 280(20): 19635-19640.

Western blot analysis of extracts using p53 (phospho- Thr18) antibody.