• Catalog Number:

  E1A7183A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 37.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  p53 (Phospho-Ser37) Antibody detects endogenous levels of p53 only when phosphorylated at Serine 37.

• References:

  Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959. Thompson T, et al.(2004) J Biol Chem. 279(51): 53015-53022. Jackson MW, et al.(2004) Oncogene. 23(25):4477-4487. Kyriakis J M, et al. (1994) Nature. 369: 156-160.

Western blot analysis of extracts from K562 cells. Line1: Using p53 (Phospho-Ser37) Antibody; Line2: Using the same antibody preincubated with synthesized phosphopeptide.