• Catalog Number:

  E1A7181A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 315 (S-S-SP-P-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  p53 (phospho-Ser315) antibody detects endogenous levels of p53 only when phosphorylated at serine 315.

• References:

  Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934. Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539. Ulrich, S. J. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 5954-5958. Pospísilová S, et al. (2004) Biochem J; 378(Pt 3): 939-47. Merrick BA, et al. (2001) Biochemistry; 40(13): 4053-66.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho- Ser315) antibody.

Western blot analysis of extracts from ovary cancer cells using p53 (phosphor-Ser315) antibody.