• Catalog Number:

  E1A7180A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of Serine 15 (P-L-SP-Q-E).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P04637

• Applications:

  IHC: 1:50~1:100 ELISA: 1:10000 WB: 1:500~1:1000 IP: Various Dilution

• Specificity Sensitivity:

  p53(Phospho-Ser15) Antibody detects endogenous levels of p53 only when phosphorylated at Serine15.

• References:

  Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823. Honda, R. et al. (1997) FEBS Lett. 420, 25-27. Hirao, A. et al. (2000) Science 287, 1824-1827.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using p53 (Phospho- Ser15) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis of the extracts from HeLa cells untreated or treated with hydroxyurea using p53 (Ab-15) Antibody and p53(Phospho-Ser15) Antibody.