• Catalog Number:

  E1A7179A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16539

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAX-interacting protein 2; MAP kinase MXI2; SAPK2A

• Specificity Sensitivity:

  P38 MAPK (Phospho-Tyr182) antibody detects endogenous levels of P38 MAPK only when phosphorylated at tyrosine 182.

• References:

  Midori Fujishiro, et al. (2001) J. Biol. Chem; 276: 19800-19806. Ji Li, et al. (2005) Circ. Res; 97: 872-879. Rocky Pramanik, et al. (2003) J. Biol. Chem; 278: 4831-4839. Tomoyuki Inoue, et al. (2005) J. Immunol; 174: 4301-4306.

• All Sites:

  Human: Tyr182; Mouse: Tyr182; Rat: Tyr182

P-peptide - + Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using P38 MAPK (Phospho-Tyr182) antibody.

Western blot analysis of extracts from K562 cells, untreated or treated with UV, using P38 MAPK (Ab-182) antibody (Line 1 and 2) and P38 MAPK (Phospho-Tyr182) antibody (Line 3 and 4).