• Catalog Number:

  E1A7178A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human P38 MAPK around the phosphorylation site of threonine 179 (E-M-TP-G-Y).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q16539

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Alter Name:

  CRK1, CSAID binding protein, CSBP, CSBP1, CSBP2, Cytokine suppressive anti- inflammatory drug binding protein, MAP kinase MXI2, MAP kinase p38, MAPK14, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38, kinase p38-alpha

• Specificity Sensitivity:

  P38 MAPK (phospho-Thr180) antibody detects endogenous levels of P38 MAPK only when phosphorylated at threonine 179.

• References:

  Jeffrey W. Ryder, J. Biol. Chem., Jan 2000; 275: 1457. Ralph G. Meyer, Eur. J. Biochem., Jan 2002; 269: 337. Jizhong Liu, Blood, Mar 2007; 10.1182. John M. Kyriakis and Joseph Avruch Physiol Rev, Apr 2001; 81: 807.

• All Sites:

  Human: Thr179; Mouse: Thr179; Rat: Thr179

Western blot analysis of extracts from Hela cells treated with TNF-α (20ng/ml, 5mins), using P38 MAPK (phospho-Thr180) antibody (Line 1 and 2).