• Catalog Number:

  E1A7177A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of Threonine 187(E-Q-TP-P-K).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P46527

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  p27Kip1(Phospho-Thr187) Antibody detects endogenous levels of p27Kip1 only when phosphorylated at Threonine 187.

• References:

  Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46. Le XF, et al. (2003) J Biol Chem. Connor MK, et al. (2003) Mol Biol Cell; 14(1): 201-13. Fujita N, et al. (2002) J Biol Chem; 277(32): 28706-13. Ishida N, et al. (2000) J Biol Chem; 275(33): 25146-54.

Western blot analysis using p27Kip1(Phospho-Thr187) Antibody. Line1: The extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with EGF. Line3: The extracts from HeLa cells treated with IFN-α.