• Catalog Number:

  E1A7175A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p21Cip1 around the phosphorylation site of threonine 145 (R-Q-TP-S-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  P38936

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  p21Cip1 (phospho-Thr145) antibody detects endogenous levels of p21Cip1 only when phosphorylated at threonine 145.

• References:

  Rössig L, et al. (2002) J Biol Chem 277(12): 9684-9689. Rössig L, et al. (2001) Mol Cell Biol 21(16): 5644-5657. Zhou BP, et al. (2001) Nat Cell Biol 3(3): 245-252.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p21Cip1 (phospho-Thr145) antibody.

Western blot analysis of extracts from HeLa cells using p21Cip1 (phospho-Thr145) antibody.