• Catalog Number:

  E1A7172A-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  NF-κB p65 (phospho-Thr254) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 254.

• References:

  Yeh PY, et al. (2004) J Biol Chem. 279(25): 26143-26148. Ryo A, et al. (2003) Mol Cell.12(6): 1413-1426. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (phospho-Thr254) antibody.

Western blot analysis of extracts from 293 cells using NF-κB p65 (phospho-Thr254) antibody.