• Catalog Number:

  E1A7169A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 276.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:3000 IHC: 1:50~1:100 ELISA: 1:20000 IP: Various Dilution

• Specificity Sensitivity:

  NF-κB p65 (Phospho-Ser276) Antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 276.

• References:

  Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

Western blot analysis of extracts from JurKat cells, using NF-κB p65 (Phospho-Ser276) antibody. Western blot analysis of extracts from HeLa cells and 3T3 cells, using NF-κB p65 (Phospho-Ser276) antibody.

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NF-κB p65 (Phospho-Ser276) antibody.