• Catalog Number:

  E1A7165A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB around the phosphorylation site of serine 869 (Y-G-SP-Q-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q00653

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  NF-κB p100/p52 (phospho-Ser869) antibody detects endogenous levels of NF-κB p100/p52 only when phosphorylated at serine 869.

• References:

  Qu Z, et al. (2004) J Biol Chem; 279(43): 44563-72. Xiao G, et al. (2001) J Biol Chem 7(2): 401-9.

P-Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p100/p52 (phospho- Ser869) antibody.

Western blot analysis of extracts from MDA-MB-435 cells using NF-κB p100/p52 (phospho-Ser869) antibody.