• Catalog Number:

  E1A7163A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of serine 311 (F-K-SP-I-M).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Alter Name:

  NFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

• Specificity Sensitivity:

  NF-κB p65 (phospho-Ser311) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at serine 311.

• References:

  Moon Kyoo Jang, J. Biol. Chem., Jun 2001; 276: 20005 - 20010. Lluís Espinosa, Mol. Biol. Cell, Feb 2003; 14: 491. Srinivas J. Kammanadiminti, Infect. Immun., Apr 2007; 75: 1765 - 1770. D. Gregory, J. Virol., Dec 2004; 78: 13582 - 13590.

• All Sites:

  Human: Ser311; Mouse: Ser311; Rat: Ser311

P-peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (phospho-Ser311) antibody.

Western blot analysis of extracts from 293 cells treated with TNF-α (20ng/ml, 30mins), using NF-κB p65 (phospho-Ser311) antibody (Line 1 and 2).