• Catalog Number:

  E1A7161A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 932 (E-T-SP-F-R).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19838

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  DNA-binding factor KBF1; EBP-1

• Specificity Sensitivity:

  NF-κB p105/p50 (Phospho-Ser932) antibody detects endogenous levels of NF-κB p105/p50 only when phosphorylated at serine 932.

• References:

  Vigo Heissmeyer, et al. (1999) The EMBO Journal; 18, 4766-4778. M. Lienhard Schmitz, et al. TRENDS in Biochemical Sciences Vol.26 No.3.

• All Sites:

  Human: Ser932; Mouse: Ser932; Rat: Ser932

Western blot analysis of extracts from Hela cells, untreated or treated with CA+TNF (20ng/ml, 10mins) , using NF-κB p105/p50 (Ab-932) antibody (Line 1 and 2) and NF-κB p105/p50 (Phospho-Ser932) antibody (Line 3 and 4).