• Catalog Number:

  E1A7160A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human p95/NBS1 around the phosphorylation site of serine 343 (S-L-SP-Q-G).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human

• SwissProtNo:

  O60934

• Applications:

  WB: 1:500~1:1000 ELISA: 1:5000

• Specificity Sensitivity:

  p95/NBS1 (phospho-Ser343) antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343.

• References:

  Hsu HL, et al (2005) Oncogene; 24(31): 4956-64. Falck J, et al. (2005) Nature; 434(7033): 605-11. Buscemi G, et al. (2004) Oncogene; 23(46): 7691-700. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101(33): 12130-5.

Western blot analysis of extracts from Jurkat cells using p95/NBS1 (Ab-343) antibody (Line 1 and 2) and p95/NBS1 (phospho-Ser343) antibody (Line 3 and 4).