• Catalog Number:

  E1A7155A-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MSK1 around the phosphorylation site of serine 376 (G-Y-SP-F-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O75582

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  90 kDa ribosomal protein S6 kinase, , polypeptide 5, KS6A5, MSPK1, Nuclear mitogen- and stress-activated protein kinase-1, RLPK, RPS6KA5, kinase MSK1, ribosomal protein S6 kinase, polypeptide V

• Specificity Sensitivity:

  MSK1 (phospho-Ser376) antibody detects endogenous levels of MSK1 only when phosphorylated at serine 376.

• References:

  Jeffrey W. Ryder, J. Biol. Chem., Jan 2000; 275: 1457. John M. Kyriakis and Joseph Avruch, Physiol Rev, Apr 2001; 81: 807. Sharron Schneider, Am J Physiol Heart Circ Physiol, Feb 2001; 280: 499. HUGO CEULEMANS and MATHIEU BOLLEN, Physiol Rev, Jan 2004; 84: 1.

• All Sites:

  Human: Ser376; Mouse: Ser375; Rat: Ser350

Western blot analysis of extracts from Hela cells treated with PMA (125ng/ml, 15mins), using MSK1 (phospho-Ser376) antibody (Line 1 and 2).