• Catalog Number:

  E1A7154A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human MKK3 around the phosphorylation site of Serine 189(V-D-SP-V-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Rat

• SwissProtNo:

  P46734

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Specificity Sensitivity:

  MKK3 (Phospho-Ser189) Antibody detects endogenous levels of MKK3 only when phosphorylated at Serine189.

• References:

  Wang W, et al. (2002)Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using MKK3 (Phospho- Ser189) Antibody. Left: Untreated; Right: Treated with synthesized phosphopeptide.

Western blot analysis of the extracts from MDA-MB- 435 cells treated or untreated with UV using MKK3 (Ab-189) Antibody and MKK3 (Phospho- Ser189) Antibody.