• Catalog Number:

  E1A7152A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1349 (E-H-YP-V-H).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08581

• Applications:

  WB: 1:500~1:3000 ELISA: 1:10000

• Alter Name:

  HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyrosine kinase, c-met, kinase Met

• Specificity Sensitivity:

  Met (phospho-Tyr1349) antibody detects endogenous levels of Met only when phosphorylated at tyrosine 1349. This antibody may cross-react with other activated protein tyrosine kinases.

• References:

  Fan S, et al. (2001) Mol Cell Biol; 21(15): 4968-4984. Schiering N, et al. (2003) Proc Natl Acad Sci USA; 100(22): 12654-12659. Plopper GE, et al. (1995) Mol Biol Cell; 6(10): 1349-1365. Ponzetto C, et al. (1993) Mol Cell Biol; 13(8): 4600-4608.

• All Sites:

  Human: Tyr1349; Mouse: Tyr1347; Rat: Tyr1350

Western blot analysis of extracts from K562 cells, using Met (phospho-Tyr1349) antibody. Western blot analysis of extracts from HepG2 cells, using Met (phospho-Tyr1349) antibody.