• Catalog Number:

  E1A7151A-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1234 (K-E-YP-Y-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P08581

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Alter Name:

  MET Hepatocyte growth factor receptor [Precursor]

• Specificity Sensitivity:

  Met (phospho-Tyr1234) antibody detects endogenous levels of Met only when phosphorylated at tyrosine 1234.

• References:

  Gherardi E. et al. (2003).Proc Natl Acad Sci U S A. 100(21): 12039-12044. Shiu SH. et al. (2001) Proc Natl Acad Sci U S A. 98(19): 10763-10768. Hughes AL. et al. (2001) Genome Res. 11(5): 771-780. Onuchic LF. et al. (2002) Am J Hum Genet. 70(5): 1305-1317.

• All Sites:

  Human: Tyr1234; Mouse: Tyr1232; Rat: Tyr1235

Western blot analysis of extracts from HepG2 cells using Met (Ab-1234) antibody (Line 1 and 2) and Met (phospho- Tyr1234) antibody (Line 3 and 4).